Sentence examples for buffer and stored for from inspiring English sources

The following day, tissues were transferred to a fresh change of the above buffer and stored for further EM processing.

The biopsy was fixed in fresh periodate-lysine-paraformaldehyde (2%) for 30 min. Tissue was then washed in 0.1 M phosphate buffer and stored for 2 3 days in 15% sucrose in 0.1 M phosphate buffer.

The supernatant was mixed with 4× SDS-PAGE loading buffer, boiled for 5 minutes and stored for subsequent analysis.

Bacterial genomic DNA was eluted in 60  μL of elution buffer and stored at −20°C for further analysis For amplification of bacterial DNA, universal bacterial primers 341F and 534R for the V3 regions of 16S rRNA genes were used and the reaction conditions were set as described by our previous studies [ 12, 13].

After 1 wash in a large volume of phosphate-buffered saline (PBS), exosomes were resuspended in PBS (50 100 µl) or in lysis buffer, and stored at −80°C for experimental analysis.

To avoid being rotten, a piece of buccal mucosa was kept in Krebs buffer and stored at 4 °C for 2 h.

After washing the spores twice with 200 µl cold TRIS-NaCl, they were resuspended in the same buffer and stored at 0°C for periods up to 48 h.

Bacterial DNA was extracted from aliquots of feces, and after the final precipitation, DNA was resuspended in 150 µL of TE buffer, and stored at −20°C for further analysis, as previously described [26].

Bacterial genomic DNA was eluted with elution buffer and stored at −20°C for further analysis.

The resulting supernatant was discarded and the membrane fraction resuspended in lysis buffer and stored at −80 °C for future use.

The bisulfite-converted DNA was resuspended in 10  μL elution buffer and stored at −80°C for BSP and MassARRAY.