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Purified plasmid DNA was diluted in TE buffer and stored at −20°C.
The DNA was re-suspended with 30 µl TE buffer and stored at −20 °C until used.
Air dried DNA pellet was dissolved in 30 µl TE buffer and stored at −20 °C until used.
Tissue samples were preserved in Chaos DNA extraction buffer and stored at room temperature.
Protein samples were diluted 1∶1 with 2X-Laemmli buffer and stored at −20°C.
Extracted DNA was resuspended in 100 µl of tris EDTA buffer and stored at −20°C.
The final protein is then dialyzed into storage buffer and stored at −20°C.
Membrane fractions were boiled for 5 min in reducing Laemmli sample buffer and stored at -70 C until use.
Finally, the pellet was resuspended in 50 µl of this buffer and stored at 4°C until use.
The resultant pellets were dissolved in the homogenizing buffer and stored at −80°C until western blotting was performed.
The fragments were immediately fixed in 2.5% glutaraldehyde and 0.1 mol/L of phosphate buffer and stored at 4°C.
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