Sentence examples for buffer and standards were from inspiring English sources

Fresh buffer and standards were prepared daily.

Briefly, the serum samples were diluted 1 2,000 with assay buffer, and standards were prepared from a pure EtOH standard (provided) by serial dilution.

Then, after washes, an incubation buffer was added to every well and standards were added into designated wells.

Coating solution was then removed, plates blocked with PBS + 10%FBS at 25°C for 2 hours, rinsed 3 times with diluted wash buffer (dH20, Tween 20, PBS 20X), and standards were then added in duplicate at 0, 62.5, 125, 250, 500, 1000, 2000, and 4000 pg/mL (diluted in X15 media).

The plasma samples were diluted in 20-fold in EIA buffer and the standards were prepared in plasma diluted 20-fold in EIA buffer.

Briefly, patient samples (1 40 dilution of 5 μl plasma in incubation buffer) or standards were added in duplicate to antibody-coated tubes (directed at pro-ANP peptide 73 90) and incubated for 30 min at room temperature.

The assays were performed according to the manufacturer's instructions, and Aβ standards were prepared in a buffer with the same composition as the samples.

a, Size-exclusion chromatogram and SDS PAGE gel of the purified PTCH1* with Amphipol A8-35 in buffer A. Molecular standards are indicated on the left side of the gels and above the elution curves.

All buffers and standard solutions were prepared using the water purified by the Milli-Q system (Millipore).

Total cell lysates were prepared in RIPA buffer, and standard immunoblotting procedures were used (Supplemental Experimental Procedures).

Tracheal aspirates and recombinant cytokine standards were diluted in blocking buffer.