Sentence examples for buffer and stained for from inspiring English sources

After incubation, the cells were washed in FACS buffer and stained for flow cytometry using rat-anti-mouse CD4 PerCP and rat-anti-mouse CD8 FITC on ice.

Briefly, mouse brain sections were washed with Tris buffer and stained for 10 min with a solution of 0.5% Thioflavin S in 50% ethanol, followed by 50% ethanol and Tris buffer, then dried and covered using Vectashield (Vector Labs, CA).

At the end of the incubation, cells washed three times with FACS buffer and stained for 20 min at 4°C in the dark with anti CD11c-PE (1∶400) in the presence of Fc block.

After the incubation time, cells were washed with FACS buffer, and stained for 20 min at 4°C with PE anti-mouse CD4, PerCP anti-mouse CD8, APC anti-mouse CD45.2 (BD Biosciences Pharmingen) or the isotype controls.

Thymocytes were harvested, washed in FACS buffer and stained for surface markers on ice.

For FACS analysis of reticulocytes, 3 5 μL of heparinized blood was washed in staining buffer and stained for CD71.

The cell mixtures were incubated for 15 min or 30 min, plated on poly-L-lysine-coated coverslips, fixed, permeabilized with cytofix and cytoperm buffer (BD Biosciences), and stained for F-actin with Rhodamine phalloidin (Sigma) and TCRVα2 with FITC-anti-TCRVα2 antibody.

On day 21 after confluency, cultured cells were fixed in 10% buffered formalin and stained for 10 min with 2% Alizarin red S (pH 4.0) (Sigma-Aldrich).

Then, cells were fixed and permeabilized by Fix/Perm buffer (eBioscience, San Diego, CA, USA) and stained for anti-forkhead box P3 (Foxp3 -PE.

Cells were washed and resuspended in a 0.5% bovine serum albumin (BSA) (Sigma) in Phosphate Buffered Saline (PBS) solution and stained for CXCR7 expression using an APC-conjugated antibody (clone 11G8) (R&D systems) or an APC-conjugated IgG1 isotype control according to manufacturer's protocol (R&D systems).

The cells were then washed three times in cacodylate buffer, rinsed in 30% methanol, and stained for 1 hour in uranyl acetate.