Sentence examples for buffer and stained as from inspiring English sources

Control cells were killed with 50% methanol in egg buffer and stained as above.

Blood samples containing spiked cell lines were then diluted using the erythrocyte lysis buffer, filtered and stained as described for patient samples.

Normal oesophageal samples were collected in 5 mM EDTA phosphate buffered saline (PBS), then processed and stained as described in online supplementary methods.

To detect total (surface and intracellular) PrPC levels, cells were fixed with 4% Roti-Histofix (Roth, Karlsruhe, Germany) at room temperature for 10 min and stained as above at room temperature using FACS buffer supplemented with 0.1% saponin.

Cells were fixed and stained as above.

Alternatively, the cells were harvested, washed twice with buffer and stained for Annexin V, PI, CD95, CD25, CD71, and CD3 as described above.

For apoptosis analysis, cells were resuspended with binding buffer and stained with Annexin V and 7-AAD for 15 min at 25 °C in the dark.

After that slides were neutralised using neutralising buffer and stained with EtBr.

The gels were run under MOPS buffer and stained with SeeBlue staining solution (Bio-Rad).

The tumor cell fraction was removed, fixed in 1% buffered PFA, and stained and analyzed as above.

Finally, cells were washed, resuspended in phosphate buffered saline and stained with DAPI, as described [66].