Sentence examples for buffer and spun at from inspiring English sources

The pellet was resuspended in 1 ml CBB buffer and spun at 105,000× g for 1 h.

The resin was washed 5 times with ice-cold lysis buffer and spun at 2000 g between washes.

Cells were freeze-thawed in lysis buffer and spun at 12,0000 g for 5 mins to remove cell debris.

Lysates were layered over a 20% sucrose cushion in the above buffer and spun at 48 000 r.p.m. for 1 h at room temperature (rotor TLA 120.1, Beckman Coulter).

Flies were homogenized in ice-cold grinding buffer (100 mM Tris-HCl, 0.15 mM NADP+) at a "concentration" of one fly per 100 µl of buffer and spun at 13,000 RPM for 5 min at 4° to pellet all solids.

The filtration unit was filled with fresh Buffer A and spun at 3220 × g for 7 min.

The supernatants were diluted with extraction buffer to 8 ml and spun at 100 000 × g, 1 hour 4°C.

After 48 h, the transfected cells were washed with phosphate-buffered saline, lysed with reporter assay lysis buffer (Promega, Madison, WI, USA) and spun at 14 000 × g to pellet cell debris.

The remaining ER proteins were mixed with an appropriate amount of 5×SDS sample loading buffer, boiled for 4 min, and spun at 13000× rpm for 5 min. The supernatant was stored at −20C for SDS-PAGE.

The extract was carefully loaded over buffer C (buffer A, but with 2 M sucrose) and spun at 12000 rpm for 60 min at 4°C.

After 48 h, cells were harvested at 4°C in cold harvest buffer and spun down at 4°C at 3000 r.p.m. for 5 min.