Sentence examples for buffer and spread on from inspiring English sources
The suspension was diluted with the same buffer and spread on an FMM agar plate containing PBSA emulsion and soybean oil with 40 μg ml-1 of chloramphenicol.
Cells were washed five times with TBST buffer and spread on glass slides for visualization under the confocal microscope (Nikon Eclipse E-800 C1) with 100 ms exposure.
For asexual development, the cells were harvested at their early growth phase (1.0 3.0 × 10 cells/mL), washed twice with KK2 buffer (20 mM K2HPO4/KH2PO4, pH 6.8), and spread on a cellulose ester membrane (48 mm in diameter) (Advantech) at a density of 2.5 × 10 cells/cm.
Biopsy specimens were oriented and spread on strips of filter paper and fixed immediately in 10% buffered formalin.
Both treatment and control samples were incubated at 37 °C for 8 h, during which time samples were collected every 2 h, appropriately diluted in phosphate buffer saline, spread on the surface of NA plates and incubated further at 37 °C for 24 h.
Cell-free viruses (same batch as used for the infection of lymphoid cells) were fixed with 4% formaldehyde (Polysciences) in 0.1 M Pipes buffer, pH 6.8, spread on glass slides, incubated at 4°C for 16 h and washed twice with PBS.
The cells were incubated for 60 min at 30°C, then washed with buffer and cell suspension was spread on agar plates containing 0.05 g/l aniline Blue after appropriate dilution.
Mitotic cells were harvested by pipetting, washed with PBS, incubated in a hypotonic buffer (20 mM Tris pH7.4, 1 mM EGTA and 40 mM KCl) and then spread on cover glass (Matsunami) by Cytospin3 (Shandon).
Spread Nutella (or Hazelnut Spread) on one half, and marshmallow fluff on the other.
The nuclei pellets were then washed once with hypotonic buffer and incubated on ice in high salt buffer for 30 min230
