Sentence examples for buffer and sonicated on from inspiring English sources

The fixed cells were lysed in the RIPA buffer and sonicated on ice with an ultrasonic sonicator (Dr. Hielscher UP 100H) at amplitude = 1 and duty cycle = 100% in 12 one-minute pulses.

16 h later, cells were pelleted, resuspended in phosphate buffer 50 mM, pH 7.4 (enzyme buffer), and sonicated on ice (three cycles of 30 sec at 70% power, Sonoplus, Bandelin Electronics, Berlin, Germany).

Cells were scraped into cold 50 mM Tris-HCl (pH 7.4) buffer and sonicated on ice.

Human neuronal cell pellets were lysed in cold RIPA buffer and sonicated on ice.

Mitoplasts were then resuspended in 450 µL hypotonic buffer and sonicated on ice to disrupt the IMM.

Next, cells were washed, resuspended in lysis buffer and sonicated on ice for 8 × 15 s steps at a 20% output in a Branson Sonicator.

For detection of fxr protein, cells were lysed in ice-cold RIPA buffer and sonicated for 45 seconds on ice.

Liver tissues were extracted and homogenized using the homogenization buffer, and sonicated for 2 min on ice.

The cell pellet was resuspended in 20 ml of the same buffer and sonicated for 15 min (15 s on, 30 s off, 70% amplitude) on ice.

For p-Thr18/Ser19-MLC2 detection, whole-cell extracts from cells on collagen were harvested in Laemmli sample buffer and sonicated for 15 s prior to centrifugation.

After wash, cell pellets were suspended in SDS Lysis Buffer and sonicated to shear DNA.