Sentence examples for buffer and sonicated for from inspiring English sources

The suspension was vortexed and centrifuged at 2,310×g for 10 min. The crude ghost membrane pellet thus obtained was resuspended in the same Tris buffer and sonicated for 3 min by ultrasound probe sonicator (Misonix, Farmingdale, NY).

The fibrils formed were diluted to 70 μ m with PBS buffer and sonicated for 4 min with a probe sonicator (Bandelin, Solopuls HD 2070) at 10%% maximum power and with 30%% cycles.

The crude ghost membrane pellet was resuspended in the same buffer and sonicated for 3 min by an ultrasound probe sonicator (Misonix, Farmingdale, NY).

Then, 300  μL of this sonicated dispersion was diluted to 3 mL in phosphate buffer and sonicated for 10 min at 15°C in N2 to obtain liposomes with a size of about 200 nm.

HEK293T cells were lysed in NP-40 lysis buffer, and sonicated for 15 times, then immunoprecipitation was performed using antibody against RAD18.

HEK293T cells were treated with H2O2 for indicated time, and then lysed in Triton-X100 lysis buffer and sonicated for 15 times.

The pellets of nuclei and membranes were lysed in 200 μl of 2× SDS sample buffer respectively, and sonicated for 10 pulses.

The remaining pellet (Rnase-resistant, purified chromatin sample (Chr)) was resuspended in SDS loading buffer, boiled, and sonicated for solubilization prior to Western analysis.

Briefly, 2 mg of DEP was suspended in 10 mL PBS buffer, vortexed, and sonicated for 30 min at 25°C using an ultrasonic processor (Bransonic®, Model 3510, Branson Ultrasonic Cooperation, Danbury, CT, USA).

B. anthracis Sterne strain was grown to an OD of 0.6 in tryptic soy broth, resuspended in 0.1 M sodium citrate buffer pH 4.7 and sonicated for 10 minutes before adding an equal volume of butanol and mixing at room temperature for 30 minutes.

Fresh human sperm samples washed with PBS (pH 7.4) were mixed with 5× SDS sample buffer, vortexed briefly, and sonicated for 5 seconds on ice.