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The plugs were washed thrice with Tris-EDTA buffer and sliced into pieces containing about 10 cells, which were inserted into a 14 × 20 cm 0.8% agarose gel (High-grade ultrapure, Bio-Rad).
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Ovaries were removed and fixed in Bouin buffer at 4°C, washed with ethanol, paraffin-embedded and sliced into 7 μm sections.
Hearts were rapidly harvested and sliced into two parts.
Hippocampi were removed and sliced into 350-μm-thick sections.
1/2 red onion, trimmed and sliced lengthwise into 1/8-inch slices.
The ventricles were then sliced into small pieces, placed in collagenase buffer and bubbled with 100%% O2 till the tissue was dissolved.
FFPE blocks were sliced into 0.5μMM thin slices and subjected to antigen retrieval with tris-EDTA buffer, endogenous peroxidase blocking, and rinsed with tris-buffered saline (TBS) containing 0.025% Triton X-100 (TBS-T).
Lungs were fixed in 4% formaldehyde, paraffin embedded, sliced into 3-μm sections, deparaffinized, and antigen unmasked in citrate buffer solution (0.01 M pH 6.0).
Immediately after removal of kidney from the dissected rats, tissues were sliced into small size (1 mm³) and fixed in 3% buffered glutaraldehyde.
All hearts were sliced into 2 mm thick transverse sections and incubated in triphenyltetrazolium chloride solution (TTC; 1% in phosphate buffer).
Subsequently, the heart was sliced into ~2 mm thick vertical slices and incubated in 2,3,5-triphenyltetrazoliumchloride (TTC, SIGMA T8877) 10 mg/ml in 0.1 M phosphate buffer (pH 7.4) for 10 min at 37 °C.
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CEO of Professional Science Editing for Scientists @ prosciediting.com