Sentence examples for buffer and sheared into from inspiring English sources

Fixed cells were washed by cold PBS, resuspended in lysis buffer, and sheared into fragments ranging between 200 and 800 bp in size on ice using 431A cup horn (Misonix, Farmingdale, NY, USA).

In brief, poly(A) mRNA was enriched from 20 μg of total RNA using oligo dT) magnetic beads, and sheared into short fragments (200 700 bp) with fragmentation buffer.

The mRNA extracted from a sample is converted to cDNA using reverse transcription and sheared into fragments.

The nuclear pellets were then washed once with CEB buffer (CEBN buffer without NP-40), pelleted, resuspended in 2 × sample buffer, and sheared by sonication.

Briefly, cells were exposed to 1% (v/v) formaldehyde for crosslinking, DNA was collected by lysis of powdered tissue in ChIP lysis buffer and sheared with sonication.

In brief, 100−400 ng of genomic DNA in 50 µL of volume of low TE buffer was sheared into fragments of approximately 300 base pairs with the Covaris S2 or E210 system (Covaris, Inc. Woburn, MA).

Total genomic DNA was extracted using a CTAB procedure [ 56] and randomly sheared into fragments for sequencing.

After washing with PBS, the cells were suspended in nuclei preparation buffer and shearing buffer, and sonicated under optimized conditions.

The stop-fix solution, hypotonic lysis buffer and shearing buffer were obtained from the ChIP-IT kit (Active Motif, Rixensart, Belgium).

Cell extracts for Western blotting were prepared by lysing cells in 1× Laemmli sample buffer and shearing genomic DNA by passing lysates through Qiashredder spin columns (Qiagen).

The mRNA was sheared into short fragments with fragmentation buffer and selected for construction of Illumina RNA-Seq libraries according to the described protocol.