Sentence examples for buffer and separated on from inspiring English sources

Purified ZGs were solubilized in lysis buffer and separated on 4%12%% 1D SDS-PAGE.

After washing five times, the beads were boiled in SDS loading buffer and separated on a SDS-PAGE gel.

The samples were boiled in sample buffer and separated on a 10% SDS-PAGE.

For the western-blot, the crude extract was incubated for 5 min at 100°C in 1× loading buffer and separated on a 12% SDS-PAGE.

Bound proteins were released from the beads by boiling in SDS sample buffer and separated on a 4 12% Tris-Glycine gel in MES buffer.

Cell pellets were lyzed in RIPA buffer and separated on a 10% SDS-polyacrylamide gel followed by transfer to a PVDF membrane (Millipore).

PCR product (8.15 µl) was mixed with 4 µl loading buffer and separated on a gel as above, by electrophoresis with TAE buffer.

Peptide samples were diluted in a PAGE sample buffer and separated on a 12% bis-Tris gel at 4°C for 2 h at 100 V.

Cell lysates were prepared at 48 hr post transfection with a standard RIPA buffer and separated on a 12% polyacrylamide-SDS gel.

Immunoprecipitates were boiled in 1× Laemelli sample buffer and separated on SDS-PAGE (10%) and transferred onto PVDF membrane (Hybond-P, Amerham).

After another 10 minutes at 30°C reactions were stopped with 10 µl stop-solution (250 mM EDTA, pH 8.0; 1 µl 32P-labelled DNA-fragment) and the samples were precipitated with ethanol, re-dissolved in 20 µl formamide-buffer and separated on denaturing polyacrylamide gels.