Sentence examples for buffer and separated in from inspiring English sources

Samples were dilute with concentrated SDS sample buffer and separated in low bis acrylamide gels as previously described [3].

Alternatively, the sample can be prepared in an acidic buffer and separated in a basic buffer.

For immunobloting, manually isolated islets from 3 month-old male mice were homogenized in 100 μl RIPA buffer and separated in SDS-PAGE gels as previously described [ 32].

For 2D electrophoresis, acetone-precipitated proteins were re-suspended in rehydration buffer and separated in first dimension (Isoelectric focusing) using 11-cm IPG strips (pH 3 10 NL Bio-Radd Laboratories, Hercules, CA, USA) as per the manufacturer's protocol.

Whole cell lysates were prepared in lysis buffer and separated in 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis followed by transfer to polyvinylidene difluoride membranes (Immobilon; Millipore, Bedford, MA, USA) as described previously (Kawakami et al, 2001b).

For measurement of mTOR and phosphorylated mTOR, aliquots of the homogenate supernatant were mixed with equal volumes of 2x SDS electrophoresis sample buffer and separated in a 3-8% NuPage Tris-acetate mini gel (Invitrogen).

Equal amount of protein (25 µg) was mixed with reducing 5× Laemmli buffer, loaded and separated in a 4 20%% precast Tris glycine SDS polyacrilamide gel (Bio-Rad, Hercules, CA, US).

The immunoprecipitate was washed five times in lysis buffer and separated into two portions, one for the RNA isolation as a sample for the following experiment and another for the Western blotting to identify the immunoprecipitation of BmAgo2.

Proteins collected from the apical surface of CNPEs were pooled and 5 ml of this sample was lyophilized overnight, suspended in 300 µl 1 X SDS-PAGE buffer without β-mercaptoethanol, and separated in a 15% SDS-PAGE gel.

To analyze the protein constituents of purified VLPs, the samples quantified by Quant-iT Protein Assay Kit (Invitrogen) were mixed with Lämmle SDS-PAGE sample buffer, boiled for 5 min, and separated in a 7.5 17.5% gradient gel.

For non-denaturing gel electrophoresis, equal amount of proteins (5 μg) were mixed with 5× loading buffer (without reducing agents and SDS), and separated in 12% native gel (without SDS) in running buffer (25 mM Tris, 192 mM glycine) for 12 h at 4°C.