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The pellet was washed twice with mitochondrial lysis buffer, dissolved in lysis buffer and saved as the mitochondria fraction.
The supernatant was diluted with 0.75 mls of IP buffer and saved as synaptosomal fraction (SF).
Sarkosyl-insoluble material was finally extracted in urea buffer and saved as the urea fraction.
The pellet was resuspended in 100 μl mitochondrial extraction buffer and saved as the mitochondrial fraction.
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The supernatants were saved as cytosolic extracts and stored at −80 °C, whereas the pellets were resuspended in 100- μl extraction buffer mix containing DTT and protease inhibitor and saved as mitochondrial fractions.
The supernatant was collected as cytosolic fraction and the pellet was resuspended in 0.1 ml Mitochondrial Extraction Buffer Mix containing DTT and protease inhibitors and then vortexed for 10 s and saved as mitochondrial fraction.
Supernatants were saved as the high salt Triton X-100 fraction, and the remaining pellets were solubilized in urea buffer (7 M urea, 2 M thiourea, 4%% 3-[ 3-cholamidopropyl dimethylammonio]-1-propanesulfonate (CHAPS), 3-[ 3-cholamidopropyl dimethylammonio]-1-propanesulfonate
Images were displayed and saved as bitmaps.
And then they were desaturated and saved as JPEG format.
The supernatant was collected and saved as the cytosolic fraction.
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CEO of Professional Science Editing for Scientists @ prosciediting.com