Sentence examples for buffer and samples were from inspiring English sources

Antigen retrieval was performed in a pressure cooker (5 minutes at max temperature) in high pH Tris-EDTA buffer and samples were cooled for 15 minutes at room temperature after pressure returned to atmospheric pressure.

The substrates were incubated with PLE for 48 h at 25 °C in borate buffer and samples were taken at predetermined intervals during the experiment.

Reactions were terminated by adding 5×SDS loading buffer and samples were separated on SDS PAGEs.

Reactions were stopped with SDS sample buffer, and samples were run on a 12.5% SDS-PAGE gel in the dark.

The pellets were eluted in protein loading buffer, and samples were vortexed and boiled for 5 mins at 95°C.

Beads were washed with increasing salt concentration buffers, chromatin eluted by SDS-bicarbonate buffer and samples were decrosslinked at 65°C in presence of high salt concentration.

Buffers and samples were injected by a nonpulsatile piston pump into the 30 μL flow cell, which was mounted on the coupling prim.

Then, wells were treated with blocking buffer, washed and samples were serially diluted in blocking buffer and incubated 2 hours at 37°C.

The transitions that maintained the same relative intensities in both the buffer and sample were considered as interference free.

Upon 5 washes with lysis buffer sample buffer was added and samples were subjected to SDS-PAGE and analysed upon blotting using tag-specific antibodies (Sigma).

Beads were then washed with NETN buffer three times, and samples were boiled with 2× SDS loading buffer, and were analyzed by immunoblot with indicated antibodies.