Sentence examples for buffer and same amounts from inspiring English sources

Protein concentrations were determined by a BCA™ protein assay kit (Thermo Scientific, Rockford, IL, USA).. Samples were heated for 10 min at 95°C with SDS-PAGE sample buffer and same amounts of proteins (30 µg) were separated by 6% SDS-PAGE separation gels, and were subsequently transblotted onto nitrocellulose membranes (Bio-Rad, Hercules, CA, USA).

The diluting buffer contained the same amounts of DTT and leupeptin but only 0.2 mM PMSF.

For negative controls, the primary antibody was replaced with blocking buffer containing the same amount of IgG from the non-immune rabbit serum.

(12) Though the crystal structure of IsdB-N2 also bound one heme per monomer, to confirm that 1 1 stoichiometry was recapitulated in solution apoprotein was titrated with increasing amounts of heme and difference spectra were generated by subtracting spectra from that of the buffer titrated with the same amounts of heme.

However, when the films were placed in pH 9.0 buffers containing the same amount of Fc(COOH 2 and glucose, the electrocatalytic response was quite small.

0.15 μl of 5% enzyme solution (urease, glucose oxidase, acetylcholinesterase, or butyrylcholinesterase) in 20 mM phosphate buffer, рН 7.4, were deposited onto one pair of electrodes and the same amount of 5% BSA in analogous buffer onto another (reference) pair of electrodes; then, the transducers underwent complete air-drying for 20 min at room temperature.

Bound peptides were washed once with wash buffer A containing the same amount of MassPrep Enhancer as in the sample and then twice with wash buffer A alone.

The microtubule stability assay was performed using 50 μl C buffer containing 62.5 μg Tubulin, pure protein (same amounts as co-sedimentation assay) and 1 mM GTP, incubated at 37 °C for 25 min. Samples for both the microtubule binding and microtubule stability assays were run on standard SDS-PAGE gels and stained with Coomassie.

And the same amount of silence.

And the same amount every month.

At specific time point, 1 ml of release buffer was taken out and replenished with same amount of new medium.