Sentence examples for buffer and run on from inspiring English sources
The cells were harvested, washed with PBS time times, lysed in 1× SDS protein loading buffer, and run on SDS-PAGE for biotin detection.
The precipitated DNA was dissolved in Tris EDTA (TE) buffer and run on to 1.2% agarose gel stained with ethidium bromide for analysis.
Beads were washed in lysing buffer, boiled in loading buffer and run on SDS-PAGE.
Whole cell lysates where boiled in SDS loading buffer and run on NuPAGE 4 12% precast Bis-Tris gels (Invitrogen).
The samples were boiled for 5 minutes in 4X non-reducing sample buffer and run on a 10% polyacrylamide gel containing gelatin (4 mg/ml) [21], [22].
The proteins were eluted from the beads by heating for 5 minute at 100°C with reducing loading buffer and run on an SDS PAGE.
The beads were resuspended in 20 μl sample buffer and run for ∼10 min on a 15% SDS polyacrylamide gel after 10 min incubation at 95°C.
After washing with lysis buffer, the beads were denatured at 95 °C in NuPAGE buffer (Invitrogen) and run on SDS-PAGE, followed by immunoblotting.
Protein samples were suspended in gel loading buffer, boiled and run on 12% SDS-PAGE.
Equal concentrations of protein was added to SDS sample buffer, boiled and run on a 6 20% acrylamide gradient gel.
