Sentence examples for buffer and resolved on from inspiring English sources

Reactions were stopped by mixing with SDS-PAGE loading buffer and resolved on SDS-PAGE gels.

Forty micrograms of protein were mixed with sample buffer and resolved on 10% SDS-PAGE.

For immunoblot analysis, 30 µg of protein was boiled in Laemmli buffer and resolved on 10% gel.

The proteins were extracted from enriched parasites by boiling in SDS sample buffer and resolved on an SDS PAGE gel.

Then, 50 µg of protein were heat denatured in SDS-PAGE sample buffer and resolved on denaturing (SDS) 10% polyacrylamide gels (SDS-PAGE).

The cytosolic and nuclear fractions were boiled in 2× Laemmli sample buffer and resolved on a 10% SDS polyacrylamide gel and probed with a phospho Erk1/2 antibody.

Reactions are divided into two equal aliquots and diluted two fold with SDS-PAGE, or native-PAGE gel loading buffers, and resolved on SDS or native gels, followed by autoradiography.

The GST beads were washed three times with GST-buffer and resolved on SDS PAGE followed by Coomassie dye staining.

Finally, the bound material was eluted from the beads using 80°C 1× SDS loading buffer (Invitrogen) and resolved on 4 12% PAGE (NuPage, Invitrogen), followed by protein band detection with Coomassie Blue R (Sigma) staining.

The eluted scFv was dialyzed against 50 mM Tris-NaCl buffer overnight and resolved on a 15 % SDS-PAGE for the analysis of its purity.

The precipitate was solubilized in SDS sample buffer (Bio-Rad) and resolved on 10% or 4 20% Tris-HCl Rgels (Bio-Rad).