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The final mitochondrial pellet was added to 100 μl of resuspension buffer and remained on ice until analyzed.
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The brain, globe, remaining ON, chiasm and proximal segment of OT were fixed in 10% buffered formalin for at least 24 h.
These results are unlikely due to slow cell surface binding and internalization of BoNT/B into SV/endosomes; as for BoNT/E, the majority of BoNT/B was already internalized into SVs/endosomes during the 5 min high-K+ buffer stimulation, and remaining toxin molecules on the cell surface after high-K+ buffer treatment were removed by extensive washing.
The red cells were lysed using lysing buffer, and the remaining granulocytes were washed.
Results demonstrated that nearly 100% of MW-treated CNTs remained on the substrates even after sonication in a buffer solution.
Mark Milligan's controversial first-half penalty was enough to keep Victory on top of the ladder with a three-point buffer and just one round remaining.
The difference between PF-MC and Buffer groups remained similar 24 hours after injury.
After exposure of the tissues was complete, tissues were gently rinsed with calcium- and magnesium-free phosphate buffered saline (PBS) until all test material was removed from each tissue insert, and any liquid remaining on the tissues was aspirated off.
After incubation and irrigation with buffer, sensor 6 remained clearly localised on the cell surface, appearing to stain the plasma membrane in a manner similar to ZIMIR with little or no internalisation of the probe over the time scale of the experiment.
(Two performances remain, on July 24 and 26).
Granulocytes located on top of erythrocytes were harvested and remaining erythrocytes were lysed (ammonium chloride buffer).
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CEO of Professional Science Editing for Scientists @ prosciediting.com