Sentence examples for buffer and read in from inspiring English sources

The medium was removed from cells, and dissolved in DMSO (DMSO 0.1% in phosphate saline buffer) and read in an ELISA micro plate reader at 570 nm; 48 h after treatment, viability was accessed by MTT assay.

Plates were then washed 3 times with wash solution, wells resuspended in dilution buffer, and read in a fluorimeter.

The plate was incubated for 15 min with agitation on a plate shaker at RT. Next, the wells were washed two times with wash buffer and read in a MAGPIX instrument (Luminex Corporation, Madison, WI, USA).

A final wash removed unbound Streptavidin-PE and the micro-particles were resuspended in buffer and read using the Luminex 100 analyzer.

After a final wash, the beads are resuspended in buffer and read on a Bio-Rad Luminex Instrument to determine the concentration of SAA.

After extensive washing, the plates were developed using alkaline phosphatase substrate (Sigma) in diethanolamine buffer, and read at A405 nm.

SPAP production was then measured by the addition of 100 µl 5 mM p-NPP per well (in diethanolamine buffer) and read on a Dynatech MRX plate reader at 405 nM.

The DFEE is able to count the single photoelectrons coming form the detector for a given time period, store the numbers in a memory buffer and read them out after a trigger, using a serial communication line.

Wells were washed three times with 1 × wash buffer, and then magnetic beads were re-suspended in 100 μl of sheath buffer and read on a Luminex® 200 machine with Milliplex Analyst software (Millipore, Billerica, MA).

Wells were washed two times with 1× wash buffer, re-suspended in 150 μl of sheath buffer, and read on a Luminex® 200 machine with Milliplex Analyst software (Millipore, Billerica, MA).

In each case, native samples were diluted into an equivalent volume of TNG buffer and read identically.