Sentence examples for buffer and ran on from inspiring English sources

Extracts were boiled with sample buffer and ran on 8 or 12% SDS-PAGE gels.

The cells were harvested, washed with PBS time times, lysed in 1× SDS protein loading buffer, and run on SDS-PAGE for biotin detection.

The precipitated DNA was dissolved in Tris EDTA (TE) buffer and run on to 1.2% agarose gel stained with ethidium bromide for analysis.

Beads were washed in lysing buffer, boiled in loading buffer and run on SDS-PAGE.

Samples were resuspended in 300 µl of staining buffer and run on a BD LSRII Flow Cytometer using BD FACSDiva Software v6.

Whole cell lysates where boiled in SDS loading buffer and run on NuPAGE 4 12% precast Bis-Tris gels (Invitrogen).

The agarose beads were washed twice with 1X IP buffer, boiled in sample treatment buffer, and run on SDS-polyacrylamide gel electrophoresis on an 8% gel.

The proteins were eluted from the beads by heating for 5 minute at 100°C with reducing loading buffer and run on an SDS PAGE.

Labeled RNAs were precipitated before mixing with the desired amount of protein in binding buffer and run on 4% native polyacrylamide gels as described previously [25].

The samples were boiled for 5 minutes in 4X non-reducing sample buffer and run on a 10% polyacrylamide gel containing gelatin (4 mg/ml) [21], [22].

Purified virions (3 ug as determined using BioRad DC protein assay) were mixed with SDS-PAGE buffer and run on a 12% SDS-PAGE gel and the gels were blotted on nitrocellulose.