Sentence examples for buffer and quantified on from inspiring English sources

The isolated DNA was finally suspended in 100 µl of elution buffer and quantified on 1  % agarose gel.

DNA was eluted with 100 l-LAE buffer and quantified on agarose gel using lambda DNA as the standard.

The supernatant was decanted, stored on ice until use, diluted 1 2 with standard diluent buffer, and quantified by colorimetric sandwich ELISA kits.

Total protein was extracted from 500 mg of onion bulb tissue, which had been frozen at −80 °C, with 500 mL of PBS buffer and quantified using a protein assay (Bio-Rad) with bovine gammaglobulin (SIGM) as the standard.

Fibronectin remaining after the extraction was then solubilized with SDS buffer and quantified by immunoblotting as described above.

Proteins were extracted by incubation with RIPA buffer and quantified by Bradford reagent.

Protein was extracted from BAT using RIPA buffer and quantified by the Bio-Rad DC protein assay.

The oligonucleotides were dissolved in the CMG buffer and quantified by A260.

The resulting extract is fractionated by HPLC on a column of silica gel, to separate the MDA-TBA adduct, which finally is eluted with methanol/phosphate buffer and quantified spectrophotometrically at 532 nm.

We eluted DNA in 2x 200 μl buffer and quantified using the QuBit dsDNA BR assay kit (Invitrogen).

DNA was dissolved in a final volume of 100 μL buffer and quantified spectrophotometrically using a BioPhotometer (Eppendorf, Hamburg, Germany).