Sentence examples for buffer and processed for from inspiring English sources

Subconfluent monolayers were gently washed with cold saline buffer and processed for immunofluorescence.

Samples were then washed with lysis buffer and processed for SDS-PAGE.

Cells were lysed using RIPA buffer and processed for immunoblotting [57] or kinase activity [58] as previously described.

Mammary glands were washed with PBS, homogenized and sonicated in a RIPA buffer and processed for western blotting.

In protocol 1 cells were grown on glass coverslips for four days, fixed with 1% glutaraldehyde in 0.1 M cacodylate buffer and processed for standard Epon-embedding [73].

The rest of the supernatant was dried out by SpeedVac (Thermo Savant, Waltham, MA), solubilized in Laemmli's buffer and processed for Western immunoblotting [31].

Resin was washed 5 times with neutralization buffer adjusted to 600 m M NaCl; biotinylated proteins were eluted directly in SDS-PAGE sample buffer, boiled, and processed for Western blot detection.

For F4/80 protein analysis, abdominal adipose tissue was collected (N = 3 WT and N = 4 N2KO) in RIPA buffer, homogenized and processed for Western analysis using the published methods [52].

Protein pellets were resuspended in Laemmli buffer, quantified and processed for western blotting.

The pancreas of 3 and 12 month-old mice with Control, pBmpr1aHet and pBmpr1aKO genotypes were fixed in 4% paraformaldehyde in phosphate saline buffer (PBS) and processed for routine haematoxylin and eosin staining and for immunofluorescence histology.

Soluble detergent extracts were either incubated with GST-SCRIB resins or Anti FLAG coupled protein G-Sepharose (GE healthcare) for 2 hr at 4°C prior to washing three times with buffer A and processed for western blot analysis.