Sentence examples for buffer and preserved at from inspiring English sources

Cells were collected by centrifugation, fixed for 15 min at room temperature in 4% glutaraldehyde in 100 mM cacodylate-HCl buffer pH 7.4, washed, resuspended in cacodylate buffer, and preserved at 4°C until imaging.

After dewetting, the tissues were fixed by 1% H2OSO4 for 2 h at 4°C and washed again, fixed with 0.1 m buffer solution of sodium cacodylate and preserved at 0°C for electron microscopy examination.

Beads were then washed in Tris Wash Buffer A containing 50 mM Tris⋅Cl pH 7.5, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, 0.5% Triton X-100, and cOmplete EDTA free, and preserved at −80°C in Tris Wash Buffer A with 10% glycerol.

The p53 proteins in stock solution were exchanged into the binding buffer (20 mM HEPES, 10% glycerol, and 150 mM NaCl [pH 7.4]) and preserved at reduced status by adding Immobilized TCEP (Tris-2′-carboxyethyl-phosphine) Disulfide Reducing Gel (Thermo Scientific).

These samples were frozen and preserved at -80°C.

Serum was separated from clotted blood and preserved at −20°C.

The supernatants were collected and preserved at -70°C.

Hippocampi were isolated and preserved at −80 °C until RNA preparation.

Serum was collected and preserved at –20°C for serologic analysis.

The muscle tissues were excised and immediately preserved at -80°C.

When WIV was stored in buffer solution, the IgG2a-dominated antibody response was preserved at all storage temperatures and storage durations tested (Table  II).