Sentence examples for buffer and prepared for from inspiring English sources

The reactions were fixed with 2% glutaraldehyde (EMS) in KHM buffer and prepared for EM [12].

Beads were washed extensively in the same buffer and prepared for western blot analysis.

Samples were incubated overnight at 4°C with end-over-end mixing, eluted with ImmunoPure® elution buffer and prepared for SDS-PAGE [21].

To assess the mutant cTnIs expression and incorporation into the sarcomere, cultured ventricular myocytes were scraped into Laemmli sample buffer and prepared for gel electrophoresis by boiling and sonication.

Precipitated immunocomplexes were released by boiling with 2 × SDS electrophoresis sample buffer and prepared for western blot analysis.

The precipitates were washed four times with lysis buffer, denatured with SDS sample buffer and prepared for WB.

The fractions containing the ribosome monomers (70S) or dimers (100S) were dialyzed at 4 °C against buffer I and prepared for electron microscopy.

After 30 days, mammary glands were excised, fixed in 4% buffered formaldehyde, and prepared for histologic evaluation.

Five micron frozen sections were washed in phosphate buffered saline and prepared for 5-bromo-4-chloro-3-inodyl β-D-galactoside (X-gal) detection followed by nuclear-fast red counterstain modified from previously described protocols[ 25].

Following washing with PBS, monolayers were fixed in 3% glutaraldehyde in 0.1% cacodylate buffer pH 7.4 and prepared for TEM as previously described [33].

To generate protein lysates, RGCs were harvested after 15 min in culture, centrifuged at 900 r.p.m. for 5 min, and the cell pellets were collected in lysis buffer (see below) and prepared for western blot analysis.