Sentence examples for buffer and prepared as from inspiring English sources

Then, the beads were rinsed three times in the immunoprecipitation buffer and prepared as immunoprecipitation samples.

For the retina lysate, the retinas were lysed in the same lysis buffer and prepared as described above for cells.

Thaw and prepare as usual.

Extraction of nuclei in buffer I (prepared as above) from viable cells results in the recovery of chromatin-free nucleoplasm.

After NZM cells were grown to about 80% confluence in the presence of serum or serum starved for 16 hours, they were washed in ice-cold PBS, lysed in radioimmunoprecipitation assay (RIPA) buffer and prepared for western blotting as previously described [ 31].

Washing buffers and antibody dilutions were provided with the kit and prepared as the kit instruction guidelines.

Sections (prepared as above) were preincubated in assay buffer (50 mmol/l Tris- HCl, pH 7.4) for 30 minutes and then incubated in the same buffer containing [3H]DAMGO (4 nM) for 60 min at room temperature as previously described [25].

But in some cases such as with 4-nitrophenyl acetate, to avoid spontaneous hydrolysis, it is important to remove the substrate from the working reagent buffer and prepare it in water as a separate starting reagent.

Fluorescence activated cell sorter (FACS) buffer was prepared as 1% PBS, 5% FBS, and 0.05% 3 M NaN3.

Lysis Buffer was prepared as 15 mM Tris HCl, pH 7.6, 150 mM NaCl, 10% Glycerol, 1% TritonX 100, 1 mM EDTA supplemented with phosphatase inhibitors (5 mM NaF, 0.4 mM Na3VO4, 1 mMNaPPi, 0.1 mM ZnCl2, 1 mM NaMOb) and protease inhibitors (0.2 mM AEBSF, 10 µM Leupeptin, 1 uM Pepstatin A and 0.8 mM Benzmaidine.

Matrix-Lysis buffers (sucrose + protease buffer, lysing buffer 8 M urea + 1% SDS, washing buffer) were prepared as previously described [ 26].