Sentence examples for buffer and pooled into from inspiring English sources

The cell pellets of rat hepatocytes are resuspended in 3-5 ml suspension buffer and pooled into one 50-ml falcon tube.

Both fractions were resuspended in Annexin V binding buffer and pooled.

The sediment in each tube was reconstituted with phosphate buffered saline (PBS) to 2.5 ml, mixed well and then pooled into one tube that served as common inoculum source for all subsequent tests.

The libraries were quantified and equally pooled into one sample.

Proteins were eluted in cleavage buffer without MESNA and pooled.

The tubes were washed by adding 0.85 ml extraction buffer, vortexed, centrifuged and pooled with supernatants.

Fractions containing high carboxypeptidase activities were dialyzed against 5 mM sodium acetate buffer and then pooled.

Pelleted ribosomal particles were resuspended in high-salt buffer and the subunits separated on 10%47%% w/v sucrose gradients by ultracentrifugation. 40S bands were carefully removed and pooled, then buffer exchanged into 50 mM HEPES pH 7.5, 10 mM MgCl2, 75 mM KCl and 0.5 mM TCEP.

All NAF collected from a single breast was pooled into 0.5 ml phosphate buffered saline and immediately placed on ice.

8 uL 0.1 M EDTA was added to each PCR product and the triplicate amplification reactions were pooled into a Clean-Up Plate (Clontech), washed 3 times with water, and eluted with 50 uL Recovery Buffer (Clontech).

The resulting cell pellets were pooled into 1 mL of lysis buffer (500 mM triethylammonium bicarbonate with 4 M urea).