Sentence examples for buffer and plated on from inspiring English sources

Immediately after treatment the biofilm cells were detached via sonication, washed with 0.85% NaCl buffer, and plated on LB-agar plates to quantify the number of viable cells by counting CFUs.

Following the transport assay, the cells were lysed in the blocking buffer and plated on ELISA plates.

Lysates were diluted appropriately in M9 buffer and plated on LB agar supplemented with gentamicin to select for B. thailandensis.

Cells were pelleted and resupended in 1 ml NP-buffer (0.15 M NaCl, 10 mM Bicine pH 8.35), pelleted a second time and resuspended in 200 µl NP-buffer and plated on to SD medium (+2% agar) lacking either leucine (prey) or tryptophane (bait).

After outgrowth overnight, cultures were spun down and resuspended in 0.1 M phosphate buffer (pH 7.1) and plated on M9 plates containing 10 g/L of glucose, 1000X A5 trace metals, and 2 g/L (JCL16 mutagenesis) or 8 g/L (AL1 mutagenesis) of AZL and incubated at 37°C for 2 3 days.

We placed each fruiting body individually in 40 μl of KK2 buffer and plated out a dilute sample of the spores with on SM/5 plates with KA.

Later the cells were suspended in potassium phosphate buffer (0.06 M, pH 6.8) and plated on nutrient agar (Kadouri et al. 2003).

For protein expression, the culture was pelleted, suspended in 150 μl of phosphate- buffered saline (PBS, pH 7.1), and plated on Luria plates containing 5 μM isopropyl-β-D-thiogalactopyranoside (IPTG) and antibiotics as described above.

The supernatants containing dissociated cardiomyocytes were washed twice with incubation buffer and the cardiomyocyte fractions separated and plated on laminin-coated plates (10 μg/ml) at 2×10 cells/cm with serum-free DMEM supplemented with 5 mM taurine, 5 mM creatine, 2 mM L-carnitine, 25 mM HEPES and 20 u/l insulin.

Additionally, 100 µL of each serial dilution of the reference strains prepared in PB buffer was plated on CVP to determine cell density.

Pellets were resuspended in H/S buffer, aliquots were plated on TSA, and grown overnight prior to quantification of the number of intracellular bacteria.