Sentence examples for buffer and plated as from inspiring English sources

The sterilized filtrate was serially diluted with phage buffer and plated as above with MBTA and M. smegmatis mc2155.

N2 and mir-83 (n4638 ) IV; mir-34 (gk437 ) X embryos were isolated using hypochlorite treatment, hatched in M9 buffer for ∼24 hr and plated as synchronized L1's on HB101-seeded NGM plates.

The cells were serially diluted in phosphate buffer and plated for screening following the similar protocol as described above.

Following the transport assay, the cells were lysed in the blocking buffer and plated on ELISA plates.

Organoids were pelleted at 1,100 rpm, washed four times with digestion buffer and then plated into standard tissue culture plates in plating medium as described previously [ 31].

Methods for the maceration of pellets in buffer and plating are detailed in [63].

For protein expression, the culture was pelleted, suspended in 150 μl of phosphate- buffered saline (PBS, pH 7.1), and plated on Luria plates containing 5 μM isopropyl-β-D-thiogalactopyranoside (IPTG) and antibiotics as described above.

Serial dilutions were performed in the same buffer, and 100 µl was plated onto BHI media plates.

In the second assay format, the diluted sera were coated to the microtitre plate and buffer or standard solution as well as the HRP conjugate were added and incubated for 2 h at 37 °C.

The pellet was washed twice with mitochondrial lysis buffer, dissolved in lysis buffer and saved as the mitochondria fraction.

After incubation, the plate was washed as above, and alkaline phosphatase-conjugated goat anti-rabbit IgG, diluted 2000-fold in assay buffer, was applied and plates were incubated for 15 min.