Sentence examples for buffer and placed into from inspiring English sources

L4 and young adult worms were selected from growth plates with a platinum worm pick, washed in NGM buffer, and placed into the fluid-filled agar microstructure.

Cells were washed three times with PBS to remove extracellular Ca2+ and unbound virus, overlaid with 25 mM HEPES buffer, and placed into a temperature-regulated 37°C environmental chamber in a ZeissLive/DuoScan confocal microscope fitted with a 100×1.4 oil objective.

Briefly, human NK cell enrichment cocktail (50 µl/ml cells) was added to PBMCs (2×107 cells/ml) in PBS+2% FBS and 1 mM EDTA and incubated at RT for 10 min. EasySep magnetic microparticles (100 µl/ml cells) were added to the cell mixture and incubated at RT for 5 min. The total suspension was brought up to 2.5 mls with buffer and placed into a magnet for 2.5 minutes.

Here, cholesterol and surfactant are dispersed together in a buffer and placed into a round bottom flask containing three necks.

To determine whether CXCL9 can induce chemoattraction of melanoma cells, three different melanoma cell lines were stained with Cell Tracker-568, resuspended in migration buffer and placed into the upper chamber of 96-well Transwells.

To this end, in vitro cultured metacestodes were washed twice with PBS and then placed in fixation solution (3%paraformaldehyde/0.05%% glutaraldehyde in 100 mM sodium cacodylate buffer (pH 6.8)) for 30 minutes at room temperature, washed in sodium cacodylate buffer and placed into 20 mM glycine in PBS for 30 minutes on ice.

Guinea pig hearts were isolated, dye-loaded, perfused with bicarbonate buffer solution, and placed into the modified slicing chamber of a vibratome (Campden7000smz).

Cultures were washed twice with phosphate buffered saline (PBS) and placed into serum- and phosphate-free HEPES-buffered Dulbecco's modified Eagle's medium (DMEM, Invitrogen, Carlsbad, CA) to prevent particle aggregation.

The spinal columns were then washed with 0.1 M phosphate buffered saline (PBS) and placed into a series of sucrose solutions (4%, 18% and 30%, Sigma) in 0.1 M PBS.

To determine the structure of the closed complex, cubes of agar with growing T. thermophilus cells were cut out and placed into buffer containing 20 mM Tris pH 7.4, 100 mM EDTA and gently agitated for 1 hr at room temperature.

Formalin-fixed and paraffin-embedded 5 µm slides were deparaffinized and placed into citrate buffer for antigen retrieval.