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Following rehydration, slides were placed in 10 mM Sodium Citrate buffer and placed in a boiling water bath for twenty minutes.
In brief, free-floating sections were rinsed in 0.1 M phosphate buffer and placed in a solution of 0.2 M boric acid (pH 9.0) at 70°C for 1 h for antigen retrieval.
The pellets were resuspended in RIPA buffer and placed in an ice bath for an additional 30 min.
The blood cell pellet was re-suspended in 500 μl STEX buffer and placed in a water bath at 45°C.
Subsequently the tissue sections were covered with streptovidin peroxidase and incubated for 30 minutes, then rinsed gently by wash buffer and placed in humid and dark box.
Briefly, the desorbate was diluted with rehydration buffer and placed in a re-swelling cassette with 18 cm pH 4 7 linear IPG strips (GE Healthcare Life Sciences) on top.
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For preparation of real sample, 1 mL blood serum is diluted in 20 mL buffer solution and placed in the electrochemical cell.
Cell pellets were suspended in 700 µl RLT lysis buffer (Qiagen) and placed in a 2-ml tube, filled with Lysing matrix B (Q BIOgene).
injection of 75 mg/kg dithizone dissolved in Li2CO3, or an equal volume of Li2CO3 buffer alone, and placed in a humidified, temperature-controlled incubator (34°C) for observation.
Sections were allowed to cool at room temperature for 20 min and then thoroughly rinsed in buffer solution and placed in the Dako Autostainer (DakoCytomation, Glostrup, Denmark) for staining.
Mice were given an i.p. injection of 75 mg/kg dithizone dissolved in Li2CO3, or an equal volume of Li2CO3 buffer alone, and placed in a humidified, temperature-controlled incubator (34°C) for observation.
More suggestions(14)
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buffer and served in
buffer and collected in
buffer and solubilized in
buffer and lysed in
buffer and postfixed in
buffer and dried in
buffer and eluted in
buffer and dehydrated in
buffer and resuspended in
buffer and suspended in
buffer and cracked in
buffer and transferred in
buffer and incubated in
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