Sentence examples for buffer and placed at from inspiring English sources
The protoplasts were further suspended in 10 ml of W5 buffer and placed at 4°C for 30 min.
Approximately 100 µl of the bloodstain extracts (prepared as described above) were combined with 100 µl of the provided extraction buffer and placed at room temperature for 3 minutes.
One microliter of cells was transferred onto different surfaces (described below) and allowed to settle for 10 min. The cells were then carefully overlaid with 200 µl of 1% methylcellulose in MOPS buffer and placed at 32°C for 1 hr before recording.
Electrode pads were wetted in Offgel IEF buffer and placed at both the ends of the strip.
After 100 passages, cultured CMT cells were harvested, fixed with 2.5% glutaraldehyde in 0.1 M cacodylate buffer, and placed at 4°C overnight.
The blood cell pellet was re-suspended in 500 μl STEX buffer and placed in a water bath at 45°C.
Their destination address is the terminal node connected to the Internet that acts as the traffic sink.Seven relay nodes, each one with a 250-MB buffer, are placed at the selected crossroads presented in Figure 2.
Worms from 5 dPH were washed free of bacteria (3 4× in S buffer), and 50 100/assay were placed at centers of 100-mm culture plates spotted at one edge with ∼5 μL n-butanol as chemo-attractant (Dosanjh et al., 2010) plus 0.34% (w/v) sodium azide to immobilize them, and S buffer plus 0.34% azide at the opposite edge as control.
Fill two small culture plates with buffer 3 and place at RT near the perfusion system.
A fairly stable pH value ∼7.4, which was fit to pH values of general biological buffers, was nicely placed at the optimum concentration of 20 mM BAS solution.
