Sentence examples for buffer and pelleted at from inspiring English sources

To remove contaminating Percoll, the purified organelles were diluted to 10 ml in R buffer, and pelleted at 100,000 g.

2×1010 transformed yeast cells were washed 3 times with cold MACS (0.5% BSA, 2 mM EDTA) buffer and pelleted at 600 xg for 10 minutes.

Parasites were eluted from the column with the same buffer and pelleted at 900 × g for 10 min. RNA was immediately isolated as described below.

SPM and MI fractions were diluted threefold with 50 mM Tris-HCl buffer and pelleted at 100,000 ×g for 1 h to remove sucrose.

The cells were washed with 1 ml of blocking buffer, mixed gently, and pelleted at 500×g for 5 min. The cells were suspended in 100 µl Cy5-conjugated Donkey-anti-chicken IgY antibody, diluted 1∶4000 in blocking buffer, incubated on ice for 1 hr, and washed as above.

Platelets, produced at the end of the culture, were isolated from supernatants by centrifugation at room temperature for 10 min at 120  g, washed with phosphate buffered saline (PBS) and pelleted at room temperature at 900  g [ 27].

Briefly, the liver, heart, kidney and lung was suspended on ice in a buffer containing 1 mM PMSF and pelleted at 10,000 g for 4 min at 4°C.

The FFPE cells 80% confluent cells were harvested by scraping, washed with 10 ml HANK's buffer (formaldehyde solution 4%, pH 7) and pelleted at 1200 r.p.m. for 5 min.

The virus particles band was collected and diluted with PBS buffer and pelleted by centrifugation at 40,000 rpm (type Beckman Ti45) for 1.5 hr at 4°C.

The mitochondrial band was collected, diluted with 2 volumes of 1 mM EDTA, 10 mM Tris-HCl, pH 7.4 buffer and pelleted by centrifugation at 20,000×g for 15 min. 20 µg of protein of each fraction was loaded on a 10% PAA-gel and separated by SDS-PAGE.

Starting with a maximum of 8 × 10 cells, the cells were washed in KK2 buffer and then pelleted at 600 × g for 3 min. The cells were resuspended in PBS with a protease inhibitor cocktail (Roche), lysed by 8 strokes of a motorized Potter Elvehjem homogenizer followed by 5 strokes through a 21-g needle, and centrifuged at 4100 × g for 32 min to get rid of nuclei and unbroken cells.