Sentence examples for buffer and mounted on from inspiring English sources

Cells were then washed, incubated for 60 min with Alexa dye-conjugated secondary antibodies, rinsed with blocking buffer and mounted on slides with ProLong Antifade medium (Molecular Probes).

Cells were covered with 60 μL of HEPES buffer and mounted on the microscope.

The sections were visualized using 3,3′-diaminobenzidine in 0.1 M Tris buffer and mounted on gelatin-coated slides.

Sections were rinsed three times for 15 min in 0.1 M phosphate buffer and mounted on gelatin-coated slides.

Hearts were rapidly excised and placed in ice-cold buffer and mounted on a constant pressure (100 mmHg) Langendorff-perfusion apparatus.

For confocal microscopy, the cells were resuspended in 200 μl of Stain Buffer and mounted on microscope slides using acrylic-based mounting medium containing DAPI (Invitrogen).

For some experiments, 50 µm-thick longitudinal floating sections of sympathetic ganglia (four stellate ganglia from two rats) were collected in 0.1M phosphate buffer (PB), and mounted on glass slides following immunohistochemistry.

Cells were subsequently washed three times in phosphate buffered saline and mounted on slides in fluorescent mounting medium (DakoCytomation, Cambridge, UK) containing DAPI (4′,6′-diamidino-2-phenlindoledichloride, 1 1000).

All coverslips were subsequently washed three times in phosphate buffered saline and mounted on slides in fluorescent mounting medium (DakoCytomation, Cambridge, UK) with DAPI (4′,6′-diamidino-2-phenlindoledichloride, 1 : 1000).

Then, the sections were visualized by reaction with 3,3′-diaminobenzidine tetrahydrochloride (Sigma) in 0.1 M Tris-HCl buffer (pH 7.2) and mounted on gelatin-coated slides.

For PSA-NCAM staining, the sections were incubated with streptavidin peroxidase complex (1 200; Vector), visualized with reaction to 3,3'-diaminobenzidinetetrachloride (Sigma, St . Louis MO) in 0.1 M Tris HCl buffer (pH 7.2) and mounted on gelatin-coated slides.