Sentence examples for buffer and mounted in from inspiring English sources

Sections were washed in wash buffer and mounted in SlowFade Gold antifade reagent with DAPI (S36938; Invitrogen).

Finally, cells were washed three times with binding buffer and mounted in 70% glycerol for microscopy analysis.

Samples were washed twice (15 minutes and 60 minutes) with McIlvaines buffer, and mounted in McIlvaines buffer with 50% glycerol.

After three washes with blocking buffer, the sections were incubated for 1 h with fluorescein isothiocyanate (FITC -labeled and Cy3-labeled secondary antibodies, washed three times with blocking buFITC -labeledted in Vectandield mounting medium (Vector Laboratories).

Coverslips were then rinsed three times with blocking buffer, incubated at room temperature for 2 h with a species-specific secondary antibody (1 200, Jackson Immunoresearch), rinsed three times in washing buffer, and mounted in Vectashield Hardset Mounting Medium (Vector Laboratories).

After washing three times, cells were then incubated in FITC-conjugated anti-mouse secondary antibody (1 : 400) dilution for 1 h at 37°C, followed by washing three times with anti-fade equilibrating buffer and mounting in anti-fade mounting medium (Molecular Probe, Eugene, OR, USA).

Slices were then rinsed 15 min in ACSF, fixed for 2 h in 4% paraformaldehyde in 0.12 M buffer phosphate and mounted in Fluoromount.

After washing in PBS, sections were covered with Cy3-conjugated donkey anti-rabbit IgG (1 : 2000; Chemicon, Darmstadt, Germany) or donkey anti-rat IgG (1 : 1000; Dianova, Hamburg, Germany) for 1 h, washed, postfixed in 4% paraformaldehyde in 0.1 M phosphate buffer, washed again, and mounted in carbonate-buffered glycerol (1 : 1, pH = 8.6).

Cover slips were then washed in this buffer for 20 min and mounted in a perfusion chamber on the microscope stage.

For DAPI and GFP visualization, ovaries were fixed in PIPES buffer as described 15 and mounted in Vectashield with DAPI Vector Labss).

The seedlings were washed two times in MTSB buffer for 5 min and mounted in AF1 anti-fadent medium (Citifluor, London, UK).