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The cells were then pelleted at 4 °C, washed twice with 10 mL ice-cold PBS, lysed in lysis buffer, and measured for radioactivity in a liquid scintillation counter, Packard TRI-CARB 1900CA liquid scintillation analyzer (Packard Instrument Company, Downers Grove, IL).
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Cell pellets were then gently resuspended by 2 ml hypotonic buffer and nuclear extracts prepared and measured for protein concentration by a spectrophotometer as described above.
At the end of the experiments, all fish were sacrificed by a lethal dose of anesthesia (500 mg/L MS-222 buffered to pH 7.4; Sigma) and measured for total length (millimeters), wet weight (milligrams), and condition factor (fish length as a percentage of body weight).
Dilution heat was subtracted by titrating tUIM or NZF into a matching buffer and was measured for each experiment.
The residue was redissolved in 400 μL radioimmunoassay (RIA) buffer and measured as described previously for E2 and 11-KT (Scott et al. 1980 , 1984.
They replaced the free tubulin dimer solution with buffer and measured the time it took for the microtubules to start to depolymerise, the time delay value.
This study showed that the purified salivary protein from 10 ml of whole saliva had the same buffer value at pH 5 as 5 mM di-hydrogenphosphate and 50% of the buffer value measured for 10 mM hydrogencarbonate.
At pH 4.5, the buffer value measured for the salivary proteins was two times higher than for 5 mM di-hydrogenphosphate and 10 mM hydrogencarbonate.
At pH 4, the buffer value measured for the salivary proteins was 6 times higher than for di-hydrogenphosphate and hydrogencarbonate where as at pH 3.5 the buffer value measured for the salivary protein was 6 times higher than for di-hydrogenphosphate and 4.5 times higher than for hydrogencarbonate.
Because heparin is primarily composed of the disaccharide IdoA 2S -(1 → 4 -GlcNS 6S), the H NMR spectrum of the IS standard in the depolymerization buffer was measured for comparison with that of the heparin depolymerization solution as shown in Fig. 5.
Briefly, the extent of TM-BBB4 cell-[I]hAβ 1-40) (0.15 μlin200 μl in ECF buffer) binding was measured for 3 min at 37°C.
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