Sentence examples for buffer and measured at from inspiring English sources

For the hydrolysis of the pNP derivatives, the corresponding volume of a pNP derivative stock solution (150 mM) was incubated for 2 20 min in appropriate buffer and measured at 410 nm in 96-well microtiter plates [ 22].

Even though all lyophilized brain extracts were dissolved in the same phosphate buffer and measured at 298 K, it was occasionally observed that small variations in spectral patterns could not be accommodated by the spectral fitting routine.

After 2 h, serosal compartment buffer was sampled and measured at 488 nm in a fluorimeter.

Samples were dialysed into 50 mM NaPi, 100 mM NaCl buffer, pH 6.5, and measured at protein concentrations between 50 and 300 μM.

The level of superoxide anion radical (O2−) was measured by NBT (nitroblue tetrazolium) reaction in TRIS buffer with coronary venous effluent and measured at 530 nm.

Color reaction was developed 30 min later after incubation with the peroxidase substrate (2,2'-azino-bis- 3-ethylbenz-thiazoline-6-sulfonic 2,2'-azino-bis- 3-ethylbenz-thiazoline-6-sulfonic 2,2'-azino-bis- 3-ethylbenz-thiazoline-6-sulfonic 2,2'-azino-bis- 3-ethylbenz-thiazoline-6-sulfonic 2,2'-azino-bis- 3-ethylbenz-thiazoline-6-sulfonic 2,2'-azino-bis- 3-ethylbenz-thiazoline-6-sulfonic 2,2'-azino-bis- 3-ethylbenz-thiazoline-6-sulfonic 2,2'-azino-bis- 3-ethylbenz-thiazoline-6-sulfonic

Cells were washed twice and measured at room temperature in buffer A. The imaging system was built around a Nikon TE2000 microscope equipped with a mercury lamp (HB0103W/2, Osram), EX436/20 excitation filter combined with DM455 dichroic mirror.

Samples were resuspended in 0.1 M phosphate buffer and absorbance measured at 260, 280 and 494 nm using a DU 800 spectrophotometer (Beckman Coulter).

Cells were washed twice with wash buffer and fluorescence measured at excitation of 485 nm and emission of 535 nm.

The reaction was stopped by adding 250 µl of a 0.1 M Na2CO3/0.1 M NaHCO3 buffer and absorbance measured at 405 nm [49].

Following a 2 h incubation and final washes, anti-GP binding to immobilized peptides was evaluated by adding MSD Read Buffer and measuring light emission at 620 nm upon electrochemical stimulation using the MSD Sector Imager 2,400.