Sentence examples for buffer and maintained at from inspiring English sources

During this period, the hearts were immersed in buffer and maintained at 39.0°C in a temperature-controlled chamber.

Briefly, VSMCs, after a 24 h serum starvation, were resuspended at 5 × 10 cells/mL into a luminometer cuvette containing phosphate buffer and maintained at 37°C for 10 min.

MCF-7 cells were cultured in Eagle's Minimum Essential Medium (EMEM), supplemented with 5% foetal calf serum (FCS), 2 mM L-glutamine, and 0.06 % Hepes buffer, and maintained at 37°C in a humid atmosphere of 5% CO2 in air.

Briefly, mice were transcardially perfused with 4 % PFA in 0.1 M phosphate buffer, pH 7.4, and maintained at 4 °C overnight.

For antigen retrieval, the slides were immersed in 10 mM sodium citrate buffer (pH 6.0) and maintained at a sub-boiling temperature for six minutes.

Antigen retrieval was carried out by immersing the slides in 10 mM sodium citrate buffer (pH 6.0) and maintained at a sub-boiling temperature for 15 minutes.

The endogenous peroxidase activity was blocked by incubation in a 3% hydrogen peroxide solution for 15 min. Antigen retrieval was carried out by immersing the slides in 10 mM sodium citrate buffer (PH 6.0) and maintained at a sub-boiling temperature for 10 min. The slides were rinsed in PBS and incubated with 5% BSA to block no-specific staining.

After 1 month of culture, the mycobacteria were harvested and adjusted to 2.5×10 cells in 100 μL of phosphate buffered saline (PBS), aliquoted and maintained at −70°C until use.

Finally, the GNP pellets were redispersed in HEPES buffer (pH 7.4, Sigma) and maintained at 4°C.

Pooled lymph nodes and spleen cells were further treated with RBC lysis buffer (BioLegend, San Diego, CA, USA) and maintained at a density of 3 5 × 10 cells/ml in 75-cm culture flasks.

Wires were separately dipped into polypropylene tubes containing 50 ml of buffer solution and were incubated and maintained at 37 °C.