Sentence examples for buffer and lysed as from inspiring English sources

The cell pellets were resuspended in 100 µl of 1X SDS-PAGE sample buffer and lysed as previously described.

Cell pellets were thawed and resuspended in TE buffer and lysed by sonication as described above.

Cells were rinsed with PBS and lysed in buffer as described above.

For western blotting, the control cells as well as cells transfected with either full-length SMAR1 construct or SMAR1-siRNA oligonucleotide were harvested, washed with 1× PBS and lysed in buffer as described earlier [ 37].

Sub-confluent cultures of untreated and bleomycin-treated cells were harvested and lysed in RIPA buffer (buffer composition as given in the Supplementary Table S1) supplemented with protease inhibitor cocktail (Sigma Aldrich).

Cells were washed with PBS and lysed in RIPA buffer as previously described [16].

Cells were then stimulated with B. pertussis at different time points and lysed in RIPA buffer as previously described [40].

HBMEC were grown to confluence and lysed in OG buffer as described above.

Following incubation with drugs, cells were collected, washed with PBS, and lysed in RIPA buffer as described above.

Cells were rinsed with ice-cold PBS 1X and lysed in RIPA buffer as previously described [ 8].

The subcellular fractions were removed in 1 ml fractions and lysed in Laemmli buffer as described above.