Sentence examples for buffer and loading on from inspiring English sources
After dissolving the received pellets in SDS-sample buffer or in DeStreak™ rehydration buffer and loading on SDS-PAGE (not shown) and 2D-PAGE (Fig. 2a), respectively, we detected more than 90% of protein in the sediment fraction.
Immunoprecipitates were then eluted in SDS 2% at 37°C for 10 min, boiled in SDS-loading buffer and loaded on SDS-PAGE.
Samples were heated at 90 °C for 3 minutes in SDS sample buffer and loaded on preparative 6% SDS-PAGE gels.
Protein samples were prepared in SDS-sample buffer and loaded on a 10% or a 15% tris-glycine SDS-polyacrylamide gel.
Biotinylated complementary RNA samples were fragmented, diluted in a formamide-containing hybridization buffer, and loaded on to the MEEBO microarray slides enclosed in custom hybridization chambers (for more information on the MEEBO oligonucleotide set please refer to http://alizadehlab.stanford.edu/).edu/
200 mg of the crude enzyme powder was then dissolved in 0.5 ml of 50 mM phosphate buffer and loaded on a Sephadex G-75 column (18.5 cm × 1.75 cm), which had been previously equilibrated with 5 mM of phosphate buffer (pH 7.0).
The phosphorylation reactions were boiled in SDS gel sample buffer and loaded on a 10% SDS-polyacrylamide gel.
After retrieval of the Dynabeads with immunoprecipitated Eg5 using a magnet, the beads were boiled in SDS gel sample buffer and loaded on a 10% SDS-polyacrylamide gel.
Embryos were anæsthetized in 3-aminobenzoic acid ethyl ester solution (Sigma), protein extracts from the whole embryos were prepared in 2X Laemmli sample buffer and loaded on SDS/PAGE.
