Sentence examples for buffer and loaded on from inspiring English sources

Immunoprecipitates were then eluted in SDS 2% at 37°C for 10 min, boiled in SDS-loading buffer and loaded on SDS-PAGE.

Protein samples were prepared in SDS-sample buffer and loaded on a 10% or a 15% tris-glycine SDS-polyacrylamide gel.

Samples were heated at 90 °C for 3 minutes in SDS sample buffer and loaded on preparative 6% SDS-PAGE gels.

Biotinylated complementary RNA samples were fragmented, diluted in a formamide-containing hybridization buffer, and loaded on to the MEEBO microarray slides enclosed in custom hybridization chambers (for more information on the MEEBO oligonucleotide set please refer to http://alizadehlab.stanford.edu/).edu/

200 mg of the crude enzyme powder was then dissolved in 0.5 ml of 50 mM phosphate buffer and loaded on a Sephadex G-75 column (18.5 cm × 1.75 cm), which had been previously equilibrated with 5 mM of phosphate buffer (pH 7.0).

The supernatant was boiled in SDS sample buffer and loaded on a 7.5% SDS-PAGE gel.

Finally, gelatinases were eluted with 20 μl zymogram loading buffer (Novex Tris Glycine SDS Sample Buffer, Life Technologies) and loaded on a 10%% gelatin gel (Life Technologies) for electrophoresis.

The beads were washed with 1% NP40 cell lysis buffer and bound proteins were solubilized in 2× NuPage LDS reducing sample buffer, boiled and loaded on 4 12% Bis-Tris Nupage gels (Invitrogen).

Pellets of the SDS-keratin complex precipitated by KCl as described above were mixed with Laemmli buffer (BioRad) and loaded on a 4-20% tris HCl gel (BioRad Criterion).

Beads were resuspended in Laemmli buffer, boiled, and loaded on an SDS polyacrylamide gel.

The final pellet was resuspended in buffer D and loaded on a heparin column with 100 mM NaCl.