Sentence examples for buffer and loaded into from inspiring English sources

Briefly, purified VLPs resuspended in endotoxin free PBS were lysed by resuspending them in equal amount of 2x SDS protein-loading buffer and loaded into a 12% SDS-polyacrylamide gel with a 4% stacking gel.

The samples were dried in a speed-vac to remove the TEA and the samples resuspended in 1X sample buffer and loaded into a single well of 11 or 15% SDS-PAGE gels.

Aliquots of whole blood were added to 96-well micro-titer plates containing the appropriate antibody-dye combinations for each assay, incubated in the dark at room temperature for 20 minutes, diluted with an appropriate buffer and loaded into Flex32™ capillary arrays (PPD) and analyzed with SurroScan™.

Samples were then boiled in Laemmli sample buffer and loaded into an Any kD Mini-protean TGX gel (Bio Rad).

The samples were supplemented with 6.25 μL of 5× gel loading buffer and loaded into a native 2% agarose gel.

Long-term storage mixes were diluted to the required concentration and volume with the provided dilution buffer and loaded into 96-well plates.

Then, 2.25 µl of pre-amplified cDNA was mixed with 2.5 µl of 2× Universal Master Mix (Applied Biosystems) and 0.25 µl of sample loading buffer (Fluidigm Corporation) and loaded into the sample inlets of the 96 × 96 Dynamic Array (Fluidigm Corporation).

Finally the antibody coupled beads were resuspended in 0.02% NaN3, 20 mM Tris buffer, pH 7.5 and loaded into a column following washing by centrifugation 10,000 × g for 30 s with 20 mM Tris buffer, pH 7.5.

Bio-Rad Polypeptide SDS-PAGE Molecular Weight Standard was prepared in the same loading buffer with DTT and loaded into the gel to provide a molecular weight standard.

Then, for every prepared library (single tube for the 8 kb library or pool of the two tubes for the 20 kb library), 12 μL of library and 4 μL of Fuel Mix (Genomic DNA Sequencing Kit) were added to 136 L of EP Buffer (Genomic DNA Sequencing Kit) and loaded into the sample loading port of the MinION® Flow Cell.

The samples were supplemented with 3.75 μL of 5× gel loading buffer (50% glycerol with bromophenol blue and xylene cyanol tracking dyes) and loaded into a native 2% agarose gel, prepared with 1× LB buffer (Faster Better Media).