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The pellets were resuspended in 100 μL of GuHCl buffer and labeled as the pellet fraction.
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The supernatants were recovered and labeled as cytoplasmic fraction while pellets were solubilized in subfractionation buffer supplemented with Triton-X-100 1%, and incubated at 4 °C for 30 min.
The strains were preprocessed and labeled as normal or abnormal.
Floating and trypsin-released cells were collected and centrifuged, washed thoroughly, resuspended in Annexin binding buffer, and labeled with Annexin V-fluorescein and propidium iodide as per manufacturer instructions (BD Pharmingen, San Jose, CA).
The fixed cells were washed, transferred to a BrdUTP labeling buffer, and labeled with BrdU overnight at room temperature.
The samples were vacuum dried, resolved in coupling buffer and labeled with Cy5 dye.
The core RNAP was then dialyzed into carbonate buffer and concentrated for labeling as described below.
CBS proteins (1 mg/mL) were diluted in modification buffer and covalently labeled.
However, two In cells were used individually for a bottom InP buffer of 50 nm and labeled CDQW(1) and CDQW(2) as shown Figure 1.
To start the EIA assay, in a 96-well plate coated with mouse anti-rabbit IgG, 100 μl of the EIA buffer was added to the wells labeled as non-specific binding (NSB); 50 μl of EIA buffer was added to the well labeled as maximum binding (B0).
A generic flow for tha packet handler modules is given in Algorithm 3. The packet sender module scans the shared circular output buffer of the packet handling modules and sends packets, which are labeled as ready, to their next hop.
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