Sentence examples for buffer and kept on from inspiring English sources
The next day, the beads were washed three times with IP buffer and kept on ice until used.
Briefly, cell pellets were suspended in lysis buffer and kept on ice for 10 minutes.
Cells were resuspended in an additional 500 µl electroporation buffer and kept on ice until use.
At certain time intervals, samples were taken, mixed with SDS sample buffer, and kept on ice.
Stock solutions of 10 mM DHA were freshly prepared in the same buffer and kept on ice.
Cells were washed, re-suspended in 0.5 ml of ice-cold Tyrode-Hepes Buffer and kept on ice until analyzed by flow cytometry.
After isolation, mitochondria were suspended in isolation buffer for cells (buffer C) and kept on ice.
Cells were resuspended in N2B27 with 1M HEPES buffer (1 50) and kept on ice prior to injection.
The protein pellets were resuspended in 100 μL of buffer F and kept on ice for Western blot analysis with anti-EspA antibodies.
The CNS and the albumen gland of randomly mating, virgin and first time mated snails were dissected in HEPES-buffered Helisoma saline then placed in 50 μl HPLC buffer (see later) and kept on ice.
