Sentence examples for buffer and kept at from inspiring English sources

The fractions were centrifuged (13,400 × g, 15 min), the precipitate washed with filter-sterilized 70% ethanol, suspended in 50 µl of TE buffer and kept at −80 °C.

Final DNA pellet was re-suspended in 50 100 μl of TE buffer and kept at 4 °C.

The purified RTs were dissolved in the RT stock buffer and kept at −30°C until use.

Each sample containing 350 µg of protein was diluted to 330 µl with rehydration buffer and kept at room temperature for 2 h.

Coral tissue was separated from the coral skeleton with a modified airgun attached to a SCUBA cylinder and subsequently stored in 20% DMSO preservation buffer and kept at −20°C until further processing.

Total RNA was eluted in 50  μL buffer and kept at −80°C until further use.

Microcosm core samples for CLSM were fixed in 2.5% glutaraldehyde Sorensen buffer phosphate for 2 h, washed four times with the same buffer phosphate, and kept at 4°C until their analysis.

The interfacial green layer was collected and dissolved in 450 μL TE buffer solution and kept at 4°C for spectrophotometer and spectrofluorometer readings and partial purification through hydrophobic interaction chromatography (HIC).

Three bilateral samples of basolateral amygdala were extracted with a blunted 0.69 mm inner diameter sample cannula (Fine Science Tools, Heidelberg, Germany), put into 700 μl Qiazol lysis buffer, vortexed and kept at −80 °C until used for further analysis.

The membrane proteins were solubilised with 7 M urea, 2 M thiourea, 40 mM Tris, 1% C7 detergent (wt/vol) and 4% 3-[ 3-cholamidopropyl dimethylammonio]-1-propanesulfonate buffer (wt/vol) and kept at -80°C before use.

Subsequently 100 μl of cell free supernatant as well as from lysis of cells were reacted with 100 μl substrate buffer (orthophenylenediamine) and kept at 37°C for 20 min, then the reaction was stopped by adding 100 μl of 2(N) H2SO4 and absorbance was measured at 492 nm [ 14]. 100 μl of macrophage cells were isolated from respective groups from 106 cells/ml dilution and then suspended in DPBS-BSA.