Sentence examples for buffer and incubated on from inspiring English sources

34 Specimens were crushed in 50 μl of the DOC lysis buffer and incubated on a shaker table at 60 °C for one hour.

Briefly, cells were washed twice with ice-cold phosphate-buffered saline (PBS), harvested in the cell extraction buffer, and incubated on ice for at least 10 min.

After incubation, the cells were gently resuspended in 75 μL RIPA buffer and incubated on ice for 30 min.

The resulting pellet was washed in PBS and lysed in 100  μl urea lysis buffer (7  M urea, 2  M thiourea, 2% CHAPS, 1% DTT, 0.8% pharmalyte, one tablet of complete protease inhibitors (per 20 ml lysis buffer) and incubated on ice for 10 min followed by centrifugation for 5 min at 13 000 r.p.m. to pellet cell debris.

The nuclei pellets were then washed once with hypotonic buffer and incubated on ice in high salt buffer for 30 min230

The mfVSG fraction was resuspended in 1 ml of ice-cold hypotonic lysis buffer and incubated on ice for 25 minutes to lyse the membranes.

The cell pellet was washed twice with ice cold PBS subsequently lysed with fresh prepared RIPA-B-buffer and incubated on ice for 15 minutes followed by centrifugation at 13.000 g for 20 minutes at 4°C.

The pellets were resuspended in buffer A and incubated on ice for 10 min.

The remaining pellet was resuspended in the RIPA cell lysis buffer, vortexed, and incubated on ice for 30 min.

After incubation, the beads were washed five times with 1 ml coupling buffer and then transferred to 0.1 M Tris HCl buffer, pH 8.0, and incubated on ice for 2 hr followed by washing with at least three cycles of buffer at alternative pHs (coupling buffer followed by 0.1 M acetic acid/sodium acetate, pH 4.0, 0.5 M NaCl).

Next, serum samples were diluted 1 100 in high-performance ELISA buffer (Sanquin, Amsterdam, the Netherlands) and incubated on the plates.