Sentence examples for buffer and incubated at from inspiring English sources

After incubation the sheets were washed in PBST buffer and incubated at room temperature for 1 hour in secondary antibody conjugated to horseradish-peroxidase (Amersham) diluted 1 : 10000.

The membrane fraction was homogenized in the solubilization buffer and incubated at 4 °C for 1 h in DDM at a final concentration of 1% DDM (w/v).

Beads were washed 4 times with NP-40 buffer, and incubated at 70 °C for 10 min after addition of 4 × LDS Sample Buffer and 2-ME.

Briefly, C. albicans yeast cells (~1 × 107/ml) were mixed with saturating amounts of the GtfB (25 μg/ml, 3 U) in adsorption buffer and incubated at 37 °C for 1 h; yeast cell were also incubated in buffer alone (without GtfB) as control.

Twenty microliters of lysozyme (50 mg/mL) and 5 μL mutanolysin (5000 u/mL) were added to the suspended cells in the TES buffer and incubated at 37°C for 30 min.

Crude enzyme was added to 0.5 mL of 1 % CMC for cellulase and xylan for xylanase in 0.05 M phosphate buffer and incubated at 50 °C for 30 min.

LrXynA was incubated in 50 mM citrate phosphate (3 7) or phosphates (6 8) buffer and incubated at 50 °C for 10 min; for pH stability, LrXynA was preincubated at 50 °C for 3 h in the same buffers.

DNA was extracted from all the samples using QIAamp DNA mini kit (Qiagen, Hilden, Germany) tissue protocol, with the following modifications: an enzymatic digestion step with 30 mg/ml lysozyme added to the tissue lysis buffer and incubated at 37°C for 1 h followed by adding 20 μl of proteinase K and 200 μl of lysis buffer and incubated at 56°C for 1 h.

All primary antibodies were diluted in blocking buffer and incubated at 4°C for 18 h.

Red blood cells (RBCs) were lysed with RBC cell lysis buffer and incubated at room temperature for three to five minutes and washed with 10% RPMI 1460.

The membrane proteins samples were mixed with SDS sample loading buffer and incubated at 37°C for 10 minutes before loading.