Sentence examples for buffer and incubate for from inspiring English sources

[? TROUBLESHOOTING]   7 Add the cells and 125I-labeled ligand into Plate A following Table 1, and adjust the total volume to 200 µL per well with cell-binding buffer and incubate for 2 h at 4 ºC.

Add the cells and 125I labeled ligand into the plates according to calculation of final concentration for each well (N = 4), and adjust the total volume to 200 µL per well with cell-binding buffer and incubate for 2 h at 4 °C.

Add the cells, 125I-labeled ligands and excess cold Protein L into Plate B following Table 2, and adjust the total volume to 200 µL per well with cell-binding buffer and incubate for 2 h at 4 ºC as the last step.

9 Add the cells and 125I labeled ligand into the plates according to calculation of final concentration for each well (N = 4), and adjust the total volume to 200 µL per well with cell-binding buffer and incubate for 2 h at 4 °C.   10 Use the vacuum manifold to remove the incubation buffer from the plates and wash 5 10 times with cell-binding buffer (100 µL/well).

Add 50 μl with a fluorescein isothiocyanate (FITC -labelled anti-mouse FITC -labellednts of goat antibody (Sigma) diluted 1:100 on ice-cold F ab' 2er and incubate fragmentsnutes of ice in a ligoatprotected environment.

Wash the membranes three times (10 min each) in TBS buffer and incubate for 1 h at room temperature with horseradish peroxidase-labeled goat anti-rabbit IgG (1 20000).

Subsequently, the gel was washed twice with Triton-X 2.5%, once with the incubation buffer and incubated for 24 h at 28 °C in the same buffer (Tris HCl 0.05 M, pH 7.5, containing CaCl2 2 mM and NaN3 0.02%).

Membranes were washed with incubation buffer and incubated for 1 hour with horseradish peroxidase-coupled goat polyclonal anti-rabbit IgG (dilution 1∶3000).

Samples were diluted in assay buffer and incubated for one hour, followed by incubation for one hour with tracer antibody.

After incubation, the cells were washed in stain buffer and incubated for 30 min at 4°C with a biotinylated sheep anti-human antibody (GE Healthcare/Amersham; 1 100).

The membrane parts were washed thoroughly with TBST buffer and incubated for 1 h with the corresponding horseradish peroxidase-conjugated IgGs (Santa Cruz Biotechnology Inc). in 3% BSA/TBST.