Sentence examples for buffer and immunoblotted for from inspiring English sources

Proteins were immobilized with Protein A sepharose beads, washed four times with lysis buffer and immunoblotted for analysis.

Total lysates using radioimmunoprecipitation assay (RIPA) buffer were prepared and immunoblotted for protein expression studies using specific primary antibodies and appropriate secondary antibodies.

In Figure 1B, upper panel, samples obtained from separation of Raji cell lysates were mixed with reducing SDS sample buffer, boiled, separated by SDS PAGE and immunoblotted for DRα.

Remaining proteins were separated by 4 × sample buffer and then analyzed by SDS-PAGE and immunoblotted for Hsp70.

Eluates were resuspended in Laemmli buffer, subjected to SDS/PAGE (10% gels) and immunoblotted for the proteins of interest.

The cells were lysed directly in Laemmli buffer and immunoblotted with HA antibody.

After detection of specific tight junctions, all membranes were stripped with Restore Western Blot Stripping Buffer, and an immunoblot for β-actin was performed to ensure equal protein loading in each lane.

For the IPs, the beads were resuspended in 50 µl of Laemmli buffer, boiled for 5 minutes and immunoblotted using an antibody against Cdk4.

After washing bound proteins were eluted with 1× SDS-PAGE sample buffer and processed for immunoblotting.

The acid-soluble proteins were boiled in SDS PAGE loading buffer and used for immunoblotting.

After 3 h at 37°C, the reaction was stopped by adding SDS loading buffer and taken for immunoblotting.