Sentence examples for buffer and immunoblotted as from inspiring English sources

Dynabeads were collected magnetically, washed, resuspended in loading buffer and immunoblotted as indicated.

Tissue was pulverized in liquid nitrogen, lysed in sample buffer and immunoblotted as previously described (De Rosa et al, 2009).

Tissue was pulverized in liquid nitrogen, lysed in sample buffer and immunoblotted as described (73).

Immunoprecipitated proteins were eluted from the beads using 100 mM glycine-HCl (pH 2.5), boiled in SDS sample buffer and immunoblotted as described above.

The cells were lysed directly in Laemmli buffer and immunoblotted with HA antibody.

For EGFR band quantification, 10 μg of A431 and Cos1 cell samples were separated on SDS-PAGE, transferred to nitrocellulose in Buffer T, and immunoblotted with anti-phosphotyrosine antibody as described above.

For epitope mapping, the Triton X-100 insoluble rTDP-43 was solublized directly in Laemmli sample buffer, and immunoblot (IB) analysis was done as described previously [ 13, 14].

Immune complexes were recovered by adding 25 μl of Protein A-Sepharose beads (Amersham Biosciences, Uppsala, Sweden), washing × 5 in lysis buffer, and resuspending in 3 × Laemmli sample buffer prior to gel electrophoresis and immunoblotting, as described above.

The pellet was resuspended in a 2x Laemmli sample buffer and proteins were resolved by SDS-PAGE and immunoblotted forBcl-2 and Bcl-xL as above.

To strip and reprobe the membranes were incubated in the stripping buffer (100 mM 2-ME, 2% SDS, 62.5 mM Tris-HCl (pH 6.7)) at 55°C for 30 min, washed, blocked and immunoblotted with an appropriate antibody as described above.

Beads were then washed five times with TNE buffer or TNE-NaCl buffer (fourth wash) and resuspended in 70 μl of SDS sample buffer to be processed by immunoblotting as previously described (27).